Journal: eLife
Article Title: Stimulation of hypothalamic oxytocin neurons suppresses colorectal cancer progression in mice
doi: 10.7554/eLife.67535
Figure Lengend Snippet: ( A–E ) The male Oxt Cre mice (2 months of age) were injected with the indicated adeno-associated viruses (AAVs) into the paraventricular nucleus (PVN), and were then treated with azoxymethane (AOM) and dextran sodium sulfate (DSS). Thereafter, clozapine-N-oxide (CNO) was i.p. injected every other day for 3 weeks. Immunohistochemical stainings for CD8α, CD4, B220, NK1.1, and CD11b of tumor tissue sections were carried out. Representative images are shown. Scale bars, 50 μm. ( F–J ) The density of immune cells in tumor tissue. n = 4 (control, B220 or control, NK1.1) or 5 (all other groups) mice per group. Data are presented as mean ± SEM. *p < 0.05, two-tailed Student’s t-test ( F ).
Article Snippet: Antibody , (Rabbit polyclonal) anti-CD8α , Bioss , Cat# bs-0648R; RRID: AB_10857537 , IHC, (1:250).
Techniques: Injection, Immunohistochemical staining, Two Tailed Test